`Seeding' with protease to optimize protein crystallization conditions inin situproteolysis
نویسندگان
چکیده
منابع مشابه
Crystallization data mining in structural genomics: using positive and negative results to optimize protein crystallization screens.
Recent efforts to collect and mine crystallization data from structural genomics (SG) consortia have led to the identification of minimal screens and novel screening strategies that can be used to streamline the crystallization process. Two groups, the Joint Center for Structural Genomics and the University of Toronto, carried out large-scale crystallization trials on different sets of bacteria...
متن کاملSystematic analysis of protein–detergent complexes applying dynamic light scattering to optimize solutions for crystallization trials
Detergents are widely used for the isolation and solubilization of membrane proteins to support crystallization and structure determination. Detergents are amphiphilic molecules that form micelles once the characteristic critical micelle concentration (CMC) is achieved and can solubilize membrane proteins by the formation of micelles around them. The results are presented of a study of micelle ...
متن کاملData mining crystallization databases: knowledge-based approaches to optimize protein crystal screens.
Protein crystallization is a major bottleneck in protein X-ray crystallography, the workhorse of most structural proteomics projects. Because the principles that govern protein crystallization are too poorly understood to allow them to be used in a strongly predictive sense, the most common crystallization strategy entails screening a wide variety of solution conditions to identify the small su...
متن کاملUsing Computational Modeling to Control and Optimize the Crystallization Process
Crystallization processes are used across the chemical, petrochemical and pharmaceutical industries. In the chemical industry, the potash and caustic industries use crystallization as a means to produce bulk chemicals and also as a separation technology for purification. The fine chemical and specialty chemical industries use crystallization processes to produce various high-value organic compo...
متن کاملIntroduction to protein crystallization.
Biological macromolecules can be crystallized by a variety of techniques, and using a wide range of reagents which produce supersaturated mother liquors. These may, in turn, be applied under different physical conditions such as temperature. The fundamental approaches to devising successful crystallization conditions and the factors that influence them are summarized here. For the Novice, it is...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Crystallographica Section F Structural Biology and Crystallization Communications
سال: 2012
ISSN: 1744-3091
DOI: 10.1107/s174430911201161x